In an HTS photoreactor, it is difficult to illuminate two samples at the same time with exactly the same amount of light, which reduces the reproducibility of the results (A).
This problem scales with the number of samples (B). In addition, samples closer to the light source may screen the light received by samples farther away (C).
When working with multiple samples, achieving uniform magnetic stirring becomes challenging. Vials located farther from the magnet’s rotational axis (A) experience weaker coupling, which results in slower or intermittent stirring, while samples positioned closer to the center (B) mix more efficiently. This radial dependence leads to concentration differences and uneven dispersion of solids.
Together, these effects make it difficult to ensure that all samples experience the same irradiation and mixing conditions. As a result, reaction outcomes can vary from vial to vial, reducing reproducibility in high-throughput experiments.